Plant regeneration from protoplast culture in flax (Linum usitatissimum)

Shoot protoplasts of four fiber flax (Linum usitatissimum) varieties (7309, 948, Belinka and Viking) were isolated and cultured. The optimal condition for higher protoplast yield 1. 8×10~6/ gFW and activity 85. 5%(c. v. 948)were from 10 day old seedings. Culture in V-KM Agroase-is-land medium led to first divisions after 3 days (c. v. 948),and after twenty days with an efficiency of 36% of divided cells and 5. 2% in plating efficiency. Plant regeneration was obtained in 7309 and Belinka on agar media B5-2,MS3 containing 0. 6mg/L 6-BA and 0.1mg/L NAA. Roots and leaves regeneration were observed in Viking and 948 respectively.