Investigation on Suitability of Pulse Duration Parameter for HeLa Cells Line Proliferation Properties towards Anti-Cancer Application
2018
In order to bring up a cells or tissue with a gene or DNA, Electroporation parameter such
as pulse duration, field strength and number of pulse plays the most important aspect. Thus, the aim
of this paper is to optimize the pulse duration parameter for HeLa cells line. This is very important
because nowadays, research on EP factors is still in limited mode. Moreover, suitable and applicable
pulse duration parameter would ensure successful treatment with least possible side effect. In this
paper, HeLa cells or known as cervical cancer cells was used to induce into a single pulse electric
field. Therefore, to achieve the aim of this study, field strength of 500V/cm was preferred and the
pulse duration was varied in the range of 100μs, 500μs, 1ms, 2ms and 5ms to obtain lowest
proliferation rate and inhibit growth of HeLa cells. The result obtained shows that HeLa cell induced
to 500V/cm with pulse duration of 100μs, induced the lowest proliferation rate (45%) as compared to
initial reading density (control) after 48 hours of culture. Thus, further research based on this work
may pave the way for an anti-cancer application through the study of the effect of pulse duration
parameter on HeLa cells.
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