ESTIMATION OF TIME SINCE DEATH BY HISTOLOGICAL EXAMINATION OF COLLECTING TUBULES IN HUMAN KIDNEY.

Background: The current research histological changes in the Collecting tubules were examined at various postmortem intervals in the deceased human. In these research histological changes of tissue after death is influenced by P.M.I. atmospheric temperature and humidity, external and internal factors. Aim: To study the time since death by post mortem histological changes of collecting tubules in human Kidney. Materials and Methods: Present research was carried out, 40 cases of deceased kidney sample in between the temperature 17.3/22.3-31.3/450C,humidity 11/36 to 75/95 and duration range 4hrs to 52.30hr. The collecting tubules were examined to establish their correlation ship with hours PMI., temperatures, humidity or time since death by studied histological (H& E, PAS staining) in Pt.J.N.M.Medical College and Dr. B.R.Ambedkar Memorial Hospital Raipur (C.G.). Result: In collecting tubules (CT) after 4hrs PMI (27.5/42.20C T) disruption of epithelium was observed in most of the place. while after 46hrs PMI( 24.3/25.90C,T) disruption of epithelium, debris in the lumen with enucleated epithelial cells  and pyknotic nuclei were seen, after 52.30hrs PMI(24.5/320C,T) disruption of epithelium, individualization of cells, debris in the lumen with pyknotic changes and enucleated cells were seen, Conclusion: Retraction of epithelium, disruption with individualization of cells, nuclear pyknosis, karyolysis and loss of tubular architecture with debris in the lumen were observed in collecting tubules. These criteria’s presented in this study could be used to determine the time after death. Keywords: Collecting tubules, P.M.I. (Post mortem interval), Medullary rays, Interstitium, Enucleated and Debris.