Reliability of manual segmentation of cornea, contact lens and tear film using a high-resolution OCT

In this study, we aimed to evaluate the intra/inter-session and intra/inter-observer variability of manual segmentation of thickness of the pre-lens tear film, contact lens (CL), post-lens tear film, epithelium, bowman’s layer, stroma and the whole cornea and CLs with a flat and a steep fit, using a commercial high-resolution spectral-domain optical coherence tomographer (OCT). Two different observers obtained three repeated measures in two separate sessions. A high correlation was found between the values obtained by the two different observers, except for the thinner layers, epithelium, and Bowman’s layer. Inter-observer analysis showed a high consistency in the measurements obtained by both observers (r2 ≥ 0.80; p   0.05). The present results su...