Anti-oxidative damage of butylated hydroxytoluene on boar spermatozoa.

In this study,the protection of BHT supplementation on spermatozoa function was evaluated with 0.2,0.4,0.8 and 1.6 mmol/L BHT during preservation of diluted and centrifugal boar semen at 15℃,then the percentages of TMS,PMI and NAR were tested at 1,2,3 and 5 d of preservation and concentration of MDA was determined at 3 and 5 d of preservation,respectively.Optimal concentration of BHT was chosen and applied to the freezing media of semen cryopreservation in 0.5 mL straws,after freezing-thawing,the percentages of TMS,PMI,NAR and Mt-MP were tested and concentration of MDA was determined.Results showed that the percentage of TMS,PMI and NAR were significantly increased than that of control in diluted and centrifugal semen(P0.05),the optimal concentration of BHT was 0.8 mmol/L and 1.6 mmol/L,respectively.Moreover,after added 0.8 mmoL/L BHT to the freezing media,the percentages of TMS,PMI,NAR and Mt-MP were increased(P0.05),and the concentration of MDA was reduced significantly than those of control(P0.05).In conclusion,addition of BHT to dilute extender or freezing media improved the quality of boar semen by inhibition of spermatic plasma membrane oxidation.