Sensitive electrochemical aptasensor for determination of sulfaquinoxaline based on AuPd NPs@UiO-66-NH2/CoSe2 and RecJf exonuclease-assisted signal amplification.

Abstract The authors designed a sensitive label-free electrochemical aptasensor for the detection of sulfaquinoxaline (SQX), including the AuPd NPs@UiO-66-NH2/CoSe2 nanocomposites and RecJf exonuclease-assisted target recycle signal amplification strategy. AuPd NPs@UiO-66-NH2/CoSe2 nanocomposite with excellent conductivity and numerous active sites was successfully synthesized to provide a favorable sensing platform and load more double-strand DNA (dsDNA) on the electrode surface. The negatively charged phosphate group of the oligonucleotide and [Fe (CN)6] 3-/4- repel each other electrostatically, resulting in very low electrical signals. In the presence of SQX, its corresponding aptamer will be released from the double-stranded structure and then digested by RecJf exonuclease, which resulted in the SQX being released to initiate the next recycling to help amplify the DPV signal. Under the optimal conditions, the peak current has a linear relationship with the logarithmic of SQX concentration in the range of 1 pg/mL∼100 ng/mL and the obtained detection limit was 0.547 pg/mL. Furthermore, the contrasted aptasensor possess reliable specificity, reproducibility and stability toward SQX, and has been applied to detect SQX in pork samples with a satisfied recovery varied from 94.40% to 95.98%.