The optimization of hybrid scaffold fabrication process in precision deposition system using design of experiments

In recent tissue engineering field, it is being reported that the fabrication of three-dimensional (3D) scaffolds having high porous and controlled internal/external architectures can give potential contributions in cell adhesion, proliferation and differentiation. To fabricate these scaffolds, various rapid prototyping technologies are being applied to. The rapid prototyping technology has made it possible to fabricate solid free-form 3D microstructures in layer-by-layer process. In this research, we introduce the development of precision deposition system, which is one of rapid prototyping technologies, and the fabrication result of scaffold using design of experiments (DOE) to optimize the deposition process. The precision deposition system required the combination of several technologies, including motion control, thermal control, pneumatic control, and CAD/CAM software. Through the organization of experimental approach using DOE, the fabrication process of hybrid scaffold, which is composed of blended poly-caprolactone, poly-lactic-co-glycolic acid and tricalcium phosphate, is established to get a uniform line width, line height and porosity efficiently.