Imaging of Lung Metastasis Tumor Mouse Model using $[^{18}F]FDG$ Small Animal PET and CT

Purpose: The purpose of this study is to image metastaic lung melanoma model with optimal pre-conditions for animal handling by using small animal PET and clinical CT. Materials and Methods: The pre-conditions for lung region tumor imaging were 16-22 h fasting and warming temperature at . Small animal PET image was obtained at 60 min postinjection of 7.4 MBq and compared pattern of uptake and glucose standard uptake value (SUVG) of lung region between Ketamine/Xylazine (Ke/Xy) and Isoflurane (Iso) anesthetized group in normal mice. Metastasis tumor mouse model to lung was established by intravenous injection of B16-F10 cells in C57BL/6 mice. In lung metastasis tumor model, image was obtained and fused with anatomical clinical CT image. Results: Average blood glucose concentration in normal mice were and in Ke/Xy group and Iso group, respectively. Ke/Xy group showed 1.5 fold higher blood glucose concentration than Iso group. Lung to Background ratio (L/B) in SUVG image was and in Ke/Xy group and Iso group, respectively. In tumor detection in lung region, image of Iso group was better than that of Ke/Xy group, because of high L/B ratio. Metastatic tumor location in small animal PET image was confirmed by fusion image using clinical CT. Conclusion: Tumor imaging in small animal lung region with small animal PET should be considered pre-conditions which fasting, warming and an anesthesia during uptake. Fused imaging with small animal PET and CT image could be useful for the detection of metastatic tumor in lung region.