Genomic context sensitivity of insulator function
2021
Compartmentalization of interactions between genomic regulatory elements and potential target genes is influenced by the binding of insulator proteins such as CTCF, which act as potent enhancer blockers when interposed between an enhancer and a promoter in a reporter assay. But only a minority of CTCF sites genome-wide function as bona fide insulators, depending on cellular and genomic context. To dissect the influence of genomic context on enhancer blocker activity, we integrated reporter constructs with promoter-only, promoter and enhancer, and enhancer blocker configurations at hundreds of thousands of genomic sites using the Sleeping Beauty transposase. Deconvolution of reporter activity by genomic position revealed strikingly different patterns of reporter function, including a compartment of enhancer blocker reporter integration sites with robust expression. The high density of integration sites permits quantitative delineation of characteristic genomic context sensitivity profiles, and their decomposition into sensitivity to both local and distant DNaseI hypersensitive sites. Furthermore, a single-cell expression approach permits direct linkage of reporters integrated into the same clonal lineage with differential endogenous gene expression, we observe that CTCF insulator activity does not completely abrogate reporter effects on endogenous gene expression. Collectively, our results lend new insight to genomic regulatory compartmentalization and its influence on the determinants of promoter-enhancer specificity.
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