Development and Validation of an GC-MS Method to Quantify Phytoconstituents by Using Entada pursaetha DC

Medicinal plants have rich source of secondary metabolites, which can be screened either qualitatively or quantitatively. It is an important procedure for qualifying the plant drug and their potentialities. The active phytomoleucles present in a plant reflect the therapeutic values of medicinal species. In order to discover a new bioactive compounds from plant resources which could become new leads or new drugs, plant extracts should be simultaneously evaluated by appropriate screening methods by either biological or pharmacological targets. For the extraction procedures, solvent extraction is commonly employed for extraction of most active principles from the plants. Entada sp. showed more than 27 different components of phytochemicals with a rich source of polysaccharides whereas polysaccharides only have major drug potential which was carried out by GC-MS analysis. In the recent studies, the bioactive components of seed of Entada pursaetha using GC-MS were investigated using Perkin-Elmer Gas Chromatography-Mass Spectrometry, while the Mass spectra of the compounds found in the extract was matched with previous literature.