Revealing microbial mechanism associated with volatile fatty acids production in anaerobic acidogenesis of waste activated sludge enhanced by freezing/thawing pretreatment

Abstract This study investigated the association of volatile fatty acid (VFA) production with microbial mechanism in waste activated sludge (WAS) anaerobic acidogenesis enhanced by freezing/thawing (F/T) pretreatment. WAS solubilization was enhanced with 955.4 ± 10.0 mg/L soluble chemical oxygen demand (SCOD) release by a 50-h F/T pretreatment at −24 °C. The highest level of VFAs (4852 ± 156 mg COD/L) was obtained after a 12-day fermentation. Moreover, phyla of Proteobacteria, Bacteroidetes, Firmicutes, and Ignavibacteriae played vital roles in VFA generation, while high genera abundance of Clostridium, Macellibacteroides, Prevotella, and Megasphaera were positively associated with high yields of short-chain (C2–C5) fatty acids. A schematic diagram was drawn to illustrate the microbial mechanism of enhanced VFA generation by F/T pretreatment during WAS fermentation. This study provides an in-depth exploration of promoting bio-resource recycling from WAS with a low-cost approach (specially in high latitudes) and bring about some new thinking on future WAS management.