Study on the relationship of sinomenine concentration between the blood and the knee joint fluid in rabbit

Objective To study the relationship of sinomenine concentration between the blood and the knee joint flu id in rabbit by microdialysis.Methods The microdialysis probes were implanted in rabbits' vein and knee joint cap sule to collect samples,the sinomenine were given by intragastric administration,transdermal administration on ab domen and transdermal administration on knee joint,the dosages were 50 mg/kg.The concentration of sinomenine in dialysate samples were detected by HPLC-MS,then the change rule of sinomenine concentration in the blood and the knee joint fluid in rabbit were observed.Results The change trend of the sinomenine concentration in blood and knee joint fluid was accordant,no matter what kinds of way to give drug.The concentration was different when the drug was given by intragastric administration and transdermal administration on abdomen(P 0.05),and there was no difference when the drug was given by transdermal administration on knee joint(P 0.05).Conclusion The chang trends of the sinomenine concentration in blood and knee joint fluid are similar,the drug concentration has related with the way of delivery and administration place.