The Use of Chemiluminescence to Detect Eicosanoids with Greater Speed and Sensitivity

The use of chemiluminescence detection for measurement of low concentration biological molecules such as eicosanoids has been developed. We report on the comparison of colorimetric enzyme generated detection with enzymatic chemiluminescent detection. We have also developed methods to indirectly attach molecules such as prostaglandin E2 (PGE2), 11s-PGF2α, and others to carrier molecules and to attach chemiluminescent Acridinium esters to these same carriers. Recently we have extended this work to attach Acridinium esters to prostaglandins using homo- and hetero-bifunctional reagents. These prostaglandin conjugates have been used to develop super sensitive and rapid immunoassays.