The use of the aggregate hemagglutination reaction and immunoenzyme analysis in determining staphylococcal enterotoxin type D

: Type D purified staphylococcal enterotoxin and anti-enterotoxic serum to it were prepared. IgG isolated from the anti-enterotoxic serum by the affinity chromatography method was used to improve the specificity and sensitivity of the aggregate hemagglutination test (AHAT) and enzyme immunoassay (EIA). The developed AHAT and EIA variants intended for the indication of type D staphylococcal enterotoxin are characterized by a high resolution ability and strict specificity. The suggested methods are simple and may be used for the rapid diagnosis of staphylococcal toxin infections.