Astragalus Trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate

I n this study, two grams of callus regenerated from stem and leaf explants of Astragalus trojanus Stev. were cultured in Woody Plant Medium (WPM) supplemented with 1 mg/L 2,4-D for four weeks and used as inoculum in order to investigate the effects of working volume and media composition. The highest biomass was obtained in 250 mL flask with astragaloside IV (1.66 µg/mg) and cycloastragenol (0.19 µg/mg) accumulation. Different concentrations of sucrose and casein hydrolysate (1 and 2 g/L) were also tested and the effect of pH was also investigated. Biomass accumulation cannot be enhanced, however, astragaloside IV and cycloastragenol content was ascended. The highest astragaloside IV (95.23 µg/mg) and cycloastragenol (5.93 mg/mg) accumulations were obtained at pH 6.8 and 2 g/L casein hydrolysate, respectively