Complete nucleotide sequence of a novel monopartite begomovirus infecting Ageratum conyzoides in China

Two isolates of a novel monopartite begomovirus were obtained from naturally infected Ageratum conyzoides plants showing typical leaf curling and enation symptoms in Sichuan Province, China. The complete DNA sequences of two isolates were determined to be 2749 nucleotides in length. Sequence analysis showed that the two isolates shared 99.5% identity, and the highest identity (89.5–89.6%) was with the DNA sequence of tomato leaf curl Hainan virus (ToLCHaiV). No other begomoviruses or satellite molecules were detected in the two samples. Based on the species demarcation criterion for the genus Begomovirus established by the Geminiviridae Study Group, the virus is a novel monopartite begomovirus, and the tentative name “ageratum leaf curl Sichuan virus” (ALCScV) is proposed. Phylogenetic analysis showed that it clustered with ToLCHaiV, and recombination analysis showed that ALCScV might have arisen by recombination between viruses related to ToLCHaiV, ageratum leaf curl virus (ALCuV), and sida leaf curl virus (SiLCuV).