Application of ELISA for Rapid Detection Aflatoxin B_1 in Milk and Dairy Products

A enzyme-linked immunosorbent assay(ELISA) was established to rapidly detect aflatoxin B1 in milk and dairy products.The sensitivity,accuracy,precision,repetitiveness and reproduction of were aflatoxin B1 detection in milk and dairy products by ELISA methodological.High performance liquid chromatography was used to verify the aflatoxin B1 by evaluated ELISA.The sensitivity was 0.03ng/ml.The recovery of standard addition was 95.0% to 104.2%.The relative average deviation and relative standard deviation of precision(RSD) was both in the range of 1.0% to 1.6%.The RSD of repetitiveness and reproduction was 3.15% and 8.21% respectively.The RSD on validation of method between HPLC and ELISA was less than 10%.The residue of aflatoxin B1 in milk and dairy products can be rapidly and accurately detected on a mass scale with ELISA method.