Iron activates NF-κB in Kupffer cells

10.1152/ajpgi.00108. 2002.— Iron exacerbates various types of liver injury in which nuclear factor (NF)-κB-driven genes are implicated. This study tested a hypothesis that iron directly elicits the signaling required for activation of NF-κB and stimulation of tumor necrosis factor (TNF)-α gene expression in Kupffer cells. Addition of Fe2+ but not Fe3+ (∼5–50 μM) to cultured rat Kupffer cells increased TNF-α release and TNF-α promoter activity in a NF-κB-dependent manner. Cu+ but not Cu2+ stimulated TNF-α protein release and promoter activity but with less potency. Fe2+ caused a disappearance of the cytosolic inhibitor κBα, a concomitant increase in nuclear p65 protein, and increased DNA binding of p50/p50 and p65/p50 without affecting activator protein-1 binding. Addition of Fe2+ to the cells resulted in an increase in electron paramagnetic resonance-detectable ·OH peaking at 15 min, preceding activation of NF-κB but coinciding with activation of inhibitor κB kinase (IKK) but not c-Jun NH2-terminal kinase. In conclusion, Fe2+ serves as a direct agonist to activate IKK, NF-κB, and TNF-α promoter activity and to induce the release of TNF-α protein by cultured Kupffer cells in a redox status-dependent manner. We propose that this finding offers a molecular basis for iron-mediated accentuation of TNF-α-dependent liver injury.