Isolation of bone morphogenetic protein from human osteosarcoma tissue

: Isolated from an osteosarcoma specimen from a 15-year-old girl and assayed by implantation, partly purified lyophilized bone morphogenetic protein (BMP) induced heterotopic ossification in the mouse quadriceps. The apparent molecular weight was 18 kDa. Osteosarcoma-derived BMP induced the same process of bone development as human BMP from normal bone matrix.