Interaction of transferrin with cefuroxime sodium

ABSTRACTThe interaction between cefuroxime sodium and transferrin in physiological buffer (pH 7.4) was investigated by spectroscopy at 298, 310, and 318 K. The analysis of data indicated that quenching mechanism of the transferrin–cefuroxime sodium system was probably static. The electrostatic force played an important role in the conjugation reaction between transferrin and cefuroxime sodium. The order of magnitude of binding constants (Ka) was 104, and the number of binding site (n) in the binary system was approximately equal to 1. Besides, the values of Hill’s coefficients were lager than 1, which indicated very weakly positive cooperativity in the transferrin–cefuroxime sodium system. The synchronous fluorescence spectra and circular dichroism spectra revealed that the microenvironment and the conformation of transferrin were changed during the binding reaction.