OVEREXPRESSION OF A20 INHIBITS LPS-INDUCIBLE ENDOTHELIAL CELLS INJURY

Objective To examine the effect of A20 on LPS inducible apoptosis and caspase-3 expression of human umbilical vein endothelial cells. Methods Using DOTAP the endothelial cells were transfected with pCDNA3 1EHA20. positive cell clones were selected with G418. The stable transfection and expression of A20 in the endothelial cells were determined by immunofluorescence analysis. The expression of the A20 gene induction by LPS was checked by RT PCR. The caspase-3 expression was checked by in situ hybridization, apoptosis of the endothelial cells was observed with flow cytometry. Results The A20 gene was able to express in the endothelial cells induced by LPS. The A20 gene transfected by DOTAP was able to inhibit 90% LPS inducible caspase-3 expression and apoptosis of the endothelial cells(P0 05).Conclusion A20 gene may be helpful for the treatment of trauma.