Determination of trichothecenes in cereals
1999
Abstract An effective method for the determination of seven trichothecenes – deoxynivalenol (DON), nivalenol (NIV), T-2 tetraol (T-24), fusarenon-X (FUS-X), diacetoxyscirpenol (DAS), T-2 toxin (T-2), HT-2 toxin (HT-2) in cereals is presented. Gel permeation chromatography on Bio-Beads S-X3 was used for clean-up of acetonitrile–methanol extract. GC–ECD was used for identification and quantification of trifluoroacetylated trichothecenes. The limit of quantitation for the method was in the range 40–200 μg/kg. Recoveries at a spiking level of 2 mg/kg ranged from 76 to 100%.
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