Identification and analysis of common pathogens causing nosocomial infections

OBJECTIVE To explore the distribution of the pathogens causing nosocomial infections and current status of drug resistance so as to guide the reasonable use of antibiotics.METHODS From Jan 2011 to Dec 2011,a total of 107 strains of pathogens were isolated from the hospital,the gram-staining smear was made for the pathogens,according the dyeing results,and the VITEK-32 automatic microorganism identification card system and the supporting bacterial identification card were adopted.The testing results were determined according to the standards developed by the United States standardization of Clinical and Laboratory Research Institute(CLSI) 2007.RESULTS Of 107 strains of pathogens isolated,the gram-negative bacteria accounted for 55.1%,and the gram-positive cocci accounted for 40.9%.The main species of the gram-positive bacteria were highly resistant to oxacillin,erythromycin,and penicillin.In addition,the coagulase-negative Staphylococcus epidermidis,Staphylococcus aureus and Enterococcus were all sensitive to vancomycin,the drug resistance rates were 5.9%,9.1%,and 11.1%,respectively.Escherichia coli,Klebsiella pneumoniae and Pseudomonas aeruginosa were highly resistant to most of the commonly used antibiotics with the drug resistance rate higher than 50.0% and showed multidrug-resistant.The drug resistance rates of the E.coli strains to ampicillin,ciprofloxacin and levofloxacin were higher than 80.0%.In addition,the study also indicated that the drug resistance rates of the E.coli and K.pneumoniae to ampicillin were 95.0% and 91.7%,respectively.CONCLUSION The gram-negative bacteria are the predominant pathogens causing nosocomial infections and show multidrug-resistant,which may be related to the unreasonable use of antibiotics in the hospital.