Muscarinic cholinergic receptor binding and electrophysiological response to acetylcholine in cultured rat caudate putamen nucleus neurons
1987
Abstract Binding sites with specificity for N-[ 3 H]methylscopolamine ([ 3 H]NMS) are present in homogenates of primary culture neurons from the rat caudate putamen nucleus (CPN). Specific binding is saturable with respect to [ 3 H]NMS. The Scatchard analysis indicates a dissociation constant of 0.6 nM and a density of 7.0 × 10 3 per cell, or 78 fmol/mg protein. In electrophysiological measurements, excitatory postsynaptic currents (EPSCs) in larger cells were increased by pressure application of acetylcholine under voltage clamp condition. The EPSCs may come from neighboring smaller cells with muscarinic acetylcholine receptors.
Keywords:
- Muscarinic acetylcholine receptor M3
- Acetylcholine
- Acetylcholine receptor
- Muscarinic acetylcholine receptor
- Muscarinic acetylcholine receptor M2
- Anatomy
- Endocrinology
- Muscarinic acetylcholine receptor M4
- Muscarinic acetylcholine receptor M1
- Excitatory postsynaptic potential
- Biology
- Internal medicine
- Voltage clamp
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