Fibroin / Alginate composite nanofiber scaffold
2013
The present invention relates to the field of processing and prepared using silk protein scaffold materials, tissue engineering and particularly to a method for preparing silk fibroin / alginate composite nanofiber scaffold. The basic step of the present invention is a fibrous degummed silk fibroin was dissolved at a concentration of 9mol / L of LiBr solution, controlling the temperature 37 ℃, time 6h, filtered after dissolution, dialysis, to obtain different concentrations by weight of air-dried yarn was concentrated fibroin solution, 500mg of sodium alginate was dissolved in 100ml of deionized water, 60 ℃ water bath to dissolve IH, magnetic stirring and then dissolved IH, made 0.5% (w / v) sodium alginate solution and the like, the present invention employs thermally induced phase separation preparation of silk fibroin / alginate composite nanofiber scaffolds, simple preparation process, no other complicated equipment, conditions easy to control, low-cost, large-scale production can be performed.
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