PREPARATION AND CHARACTERIZATION OF MACROPOROUS HYDROPHOBIC INTERACTION MONOLITHIC COLUMN

Ethylene dimethacrylate and glycidyl methacrylate were employed to prepare monolithic column followed by modification with n-butylamine to afford macroporous hydrophobic interaction monolith,in which n-heptanes was used as porogen solvent.Mercury intrusion analysis showed that 65.7% pores of the monolith had the diameter larger than 500nm,which contributed the lower pressure drops of 10.9MPa even at the velocity of 2890cm/h.The recovery of BSA on the hydrophobic interaction monolith was higher than 90% and dynamic binding capacity was 14.4mg BSA/g media which almost kept as the constant with various operating velocities of mobile phase.Base-line separation of cytochrome C,Rnase A,lysozyme and chicken egg white albumin was achieved within 3min at flow rate of 1445cm/h and the result was the same as that operated at flow rate of 361cm/h.These indicated the hydrophobic interaction monolith column was promising for fast separation and purification of proteins.