The predominance of a naive T helper cell subset in the immune response of experimental acute pancreatitis

Abstract Introduction In necrotizing acute pancreatitis (NAP), systemic inflammatory response syndrome (SIRS) and the compensatory anti-inflammatory response syndrome (CARS) decide overall outcome and mortality. In patients, low lymphocyte counts were found, but T-helper cells seemed to conversely increase. Our aim was to further categorize T-helper cells within the context of NAP induced SIRS and CARS. Methods NAP was induced by injection of sodium-taurocholate into the common bile duct of male BALB/c mice; sham treated animals received saline infusion. The animals were sacrificed at 6, 12, 24 and 48 h later. Lymphocytes from blood, liver and spleen were isolated and examined by flow cytometry. Staining was performed for CD4, CD8, CD19, CD45RB, CD25, CD69, and CD152. CD4 + cells were sorted for their CD45RB expression and sought for gene regulation associated to T H1 /T H2 cells by quantitative RT-PCR. Results In NAP, CD4 + was solely increased in all compartments. CD8 + remained without substantial alterations. CD45RB showed significant expression in RB high in T-helper cells, confirmed by the CD45RB high/low ratio (Liver, 24 h: NAP 2.2, SHAM 0.6; p  high and −low cells were not associated to patterns of T H1 /T H2 expression. In NAP, CCR4 expression was significantly decreased within RB high cells (fold change: 0.04, p  Conclusion T-helper cells increase in NAP, leaning towards CD45RB high expression. They resemble naive T-cells, in which NAP leads to expression profiles associated with an innate immune response. This suggests new findings in immunological pathomechanisms of NAP.