Expressions of HSP and p53 protein in gastric mucosa of atrophic gastritis induced by high-salt hot water in rats

AIM:To study the relationship between hot high-salt diet and chronic atrophic gastritis (CAG) and gastric cancer, and detect the expression of HSP60, HSP 70 and HSP 90 α and p53 protein in the rat gastric mucosa of atrophic gastritis induced by high-salt hot water. METHODS:Rat CAG models were firstly established by intragastrical feeding with 15 % 55 °C salt water for 12 weeks. Some rats were killed to detect HSP60,HSP 70,HSP 90 α and p53 protein every 4 weeks by immunohistochemical method in the control group (intragastrically fed with 25 °C distilled water for 12 weeks) and the experimental group. RERULTS:The expression of HSP60,HSP70 and HSP90 α was low in gastric mucosal tissue cells of rats at 4 weeks and 8 weeks. There was no significant difference between the experimental and the control group (P >0.05). The expression rates were significantly higher in the rats of experimental group at 12 weeks when CAG had formed than those in the control group (P <0.01). The expression rates of HSP 70 were the highest (33 %) among these proteins, the expression rates of HSP90 α were moderate (24 %), and the expression rates of HSP60 were the lowest (6 %). The p53 protein was not detected in normal group rats as well as in rats of the model group at 4 weeks and 8 weeks, but it was detected at 12 weeks in the CAG rats of the model group with an expression rate of 15.5 %,which was significantly associated with the expression of HSP70 and HSP90 α, especially, the former which had no association with the expression of HSP60. CONCLUSION:Long time high-salt hot diet can induce CAG, and may even promote the development of gastric cancer. HSP70/HSP90 α and p53 proteins might have combined effect in the development from CAG to gastric cancer. Jia CH,Zhang L,Zhang LX,Xu JR,Jiang M,Zhang NX,Cao GZ.Expressions of HSP and p53 protein in gastric mucosa of atrophic gastritis induced by high-salt hot water in rats. Shijie Huaren Xiaohua Zazhi 2003;11(1):29-32