A Effect of Heavy Metal to Toxicity of Triclosan Focused on Vibrio fischeri Assay

The purpose of this study is to evaluate effect of heavy metals (i.e., , , , , , and ) to toxicity of Triclosan as binary mixture. The individual toxicity and combined toxic effects of Triclosan with heavy metals were evaluated by Vibrio fischeri assay. In individual toxicity, the was found to be most toxic followed by Triclosan, , , , , and , respectively. To evaluate combined toxic effect, correlation analysis of `predicted value` calculated by Concentration addition (CA) model and Independent action (IA) model with `experimental value` were performed based on the toxicity of individual compound. As a result, all of the combinations showed that IA model were more correlated with experimental value than CA model. On the basis of the median effect concentration of combination () predicted by IA model, experimental of Triclosan + Cu, Triclosan + Zn, Triclosan + Pb, Triclosan + Hg, Triclosan + Cd, and Triclosan + Cr were 191%, 226%, 138%, 137%, 209%, and 138% of predicted by IA model, respectively, indicating that all of the combinations produced antagonistic effect.