PhoP deletion of avian Pasteurella killed attenuated strain and its construction method and application

The present invention discloses a phoP deletion of avian Pasteurella multocida attenuated strain and its construction method and application, belong to the technical field of biological engineering. Classification of the attenuated strain named Pasteurella multocida subsp.multocida str pm0818 ΔphoP, preserved in China Center for Type Culture Collection under accession number CCTCC NO: M 2014522, preservation time for the October 29, 2014. The present invention further provides a process for the preparation of the attenuated strain, the method of homologous recombination mediated by suicide plasmid based on the phoP gene knock removed to obtain the attenuated strain, the lower the toxicity of the attenuated strain, may be used living vaccine fowl cholera prevention. The present invention also announced the application of the attenuated strain in the vaccine.