The micro-ELISA sandwich technique for the quantitation of Leishmania donovani soluble antigen.

A micro-plate double antigen sandwich method is described for the quantitation of Leishmania donovani soluble antigen. Antisera were produced in rabbits by intravenous inoculation of L. donovani promastigotes. The immunoglobulin fraction of rabbit antisera containing specific antibodies was used for coating the plates and for labelling with alkaline phosphatase. The technique is reproducible and has a sensitivity limit of 10 μg soluble antigen/ml.