Copy number variations of DNA repair genes and the age-related cataract: Jiangsu Eye Study.

PURPOSE. DNA damage is critical in the pathogenesis of agerelated cataract (ARC). This study examined the association of copy number variations (CNVs) of DNA repair genes with susceptibility to ARC in the Han Chinese. METHODS. Study participants were from the population-based Jiangsu Eye Study, which includes 780 ARC patients and 525 controls. DNA was extracted from blood for copy number (CN) assays using RT-PCR. The Comet assay was to assess DNA damage in peripheral lymphocytes. RESULTS. Novel CNV was detected in WRN. Initial analyses found that CN ¼ 3þ for WRN had an increased risk of ARC (odds ratio [OR] ¼ 1.88, P ¼ 0.02); CN ¼ 1f orHSF4 had an increased risk of ARC (OR ¼4.09, P ¼0.004). CN ¼3þfor WRN was associated with nuclear and posterior subcapsular cataract (OR ¼ 2.06, P ¼ 0.02; OR ¼ 3.72, P ¼ 0.02). CN ¼ 1f orHSF4 was associated with nuclear and posterior subcapsular cataract (OR ¼5.73, P ¼0.001; OR ¼6.80, P ¼0.01). The combination WRN and HSF4 CNVs markedly increased the risk of ARC; the OR was increased from 2.63 by HSF4 alone to 6.80 by combined WRN and HSF4 CNVs. However, after multiple testing correction, only HSF4 CNV was associated with ARC overall and with nuclear and posterior subcapsular cataract as well. The DNA damage in lymphocytes from ARC patients was significantly higher when compared to normal controls. CONCLUSIONS. HSF4 and WRN CNVs might be involved in ARC pathogenesis in the Han Chinese. These findings suggest the importance of DNA repair in ARC susceptibility and distinct risk factors in ARC subtypes. (Invest Ophthalmol Vis Sci. 2013; 54:932‐938) DOI:10.1167/iovs.12-10948