The regime of Ca2+ oscillations at fertilization is determined by the culture media formulation

Several reports have shown that incubation media used for in-vitro fertilization impacts adult phenotype. However, it is still difficult to identify the critical composition of the culture media that modulates the development. Here we develop a new quantitative method that makes it possible to establish functional linkage between the composition of the medium, the regime of Ca2+ signaling and mitochondrial activity during fertilization, the miRNA expression and the post-natal growth.