La phosphoglycolate phosphatase des feuilles de haricot: Deux formes isofonctionnelles☆

Abstract 2-Phosphoglycollate phosphohydrolase (EC 3.1.3.18) was isolated and partially purified from leaves of Phaseolus vulgaris L. by acetone fractionation followed by chromatography on DEAE-cellulose. Two chromatographically stable forms could be separated by stepwise elution from DEAE-cellulose; they have been resolved by electrofocusing (pI 4.2 and 5.5) and exhibited two protein bands ( R itf s 0.68 and 0.82) on polyacrylamide gel electrophoresis. These data suggest the isoenzymatic nature of the two forms which are specific for phosphoglycollate.