Single Chain Antibodies from Chicken Immune Phage Display Libraries: Reagents for Regenerative Medicine Applications and Targeting Devices for Anti- Metastasis Therapeutics

Introduction We have generated a panel of high affinity scFv antibodies using chicken immune phage display technology that bind to the primary cilium of a quiescent population of lineage CD45 Mesenchymal Stem Cells (MSCs) that reside in human bone marrow (hbm). This MSC subset can be readily isolated using Miltenyi MACs systems, expanded rapidly in culture and can differentiate into bone, cartilage and fat tissue, and are therefore of particular interest in regenerative cell therapy. This panel of recombinant antibodies also bind to a small number of CD14 MCSF receptor, RANK CD51/61 osteoclasts precursors (OPCs) circulating in peripheral blood.