A Fluorescent Intercalator Displacement Assay for Establishing DNA Binding Selectivity and Affinity

Winston C. Tse,Dale L. Boger

A Fluorescent Intercalator Displacement Assay for Establishing DNA Binding Selectivity and Affinity

2004

Winston C. Tse
Dale L. Boger

A protocol for a fluorescent intercalator displacement (FID) assay useful for establishing DNA binding selectivity, affinity, stoichiometry, and binding site size, and for distinguishing modes of DNA binding is presented.

Keywords:

DNA
Intercalation (chemistry)
Binding selectivity
Fluorescence
Nucleic acid
Organic chemistry
Chemistry

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