Gene Combination of CD44 rs187116, CD133 rs2240688, NF-κB1 rs28362491 and GSTM1 Deletion as a Potential Biomarker in Risk Prediction of Breast Cancer in Lower Northern Thailand

Background: Biomarkers play an important role in oncology, including risk assessment, treatment prediction, andmonitoring the progression of disease. In breast cancer, many genes are used as biomarkers. Since, several SNP variationsof hallmark – related genes have been reported to be of value in risk prediction in various cancers and populations, somegenetic polymorphism loci were combined and reported as biomarkers for use in the risk assessment of breast cancerin Thai people. Methods: Twelve cancer gene hallmarks (15 polymorphic loci) were selected and genotyped in 184breast cancer patients and 176 healthy individuals in Phitsanulok, Thailand. Results: AA genotype of CD44 rs187116(c.67+4883G>A), the C allele of CD133 rs2240688 (c.*667A>C), the *2 allele (4 bp deletion) of NF-κB1 rs28362491and the homozygous null allele genotype of GSTM1 were significantly associated with an increased risk of breast cancer(p A), CD133rs2240688 (c.*667A>C), NF-κB1 rs28362491 and GSTM1 homozygous null allele genotype might be associated withan increased risk of breast cancer, and this gene combination could possibly be used as biomarkers for risk prediction,which would be of benefit in planning health surveillance and cancer prevention.