Transcriptome and secretome analyses of the adaptive response of Pseudomonas aeruginosa to suboptimal growth temperature
2009
Pseudomonas aeruginosa is an opportunistic pathogen involved in several diseases, including cystic fibrosis
and nosocomial infections. Although the behavior of this bacterium at 37°C has been intensively studied, little is known about
its capacity to adapt and survive at suboptimal temperatures, such as those encountered in hospitals. In this work, transcriptomic
and proteomic analyses were used to identify factors that allow P. aeruginosa to become established at room temperature
(close to 25°C) and thus facilitate host infections. Since the virulence of this pathogen is multifactorial and dependent on
the extracellular release of toxins and degradative enzymes targeted to the host by several secretory systems, the study focused
on genes activated at 25°C, namely, those encoding either components of the secretory machinery or secreted proteins. These
observations were enhanced by 2D-PAGE analyses, which showed that the production of effectors from type I and type II
secretion systems (respectively, proteases AprA and PrpL) and of a hemolysin co-regulated protein (Hcp) related to the type
VI secretion system was specifically stimulated when the growth temperature was lowered from 37 to 25°C. The results provide
a fundamental basis for investigating the processes that allow P. aeruginosa to adapt to suboptimal growth temperatures
and which thereby promote nosocomial infection. [Int Microbiol 2009; 12(1):7-12]
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
26
References
29
Citations
NaN
KQI