Overexpression of GSK-3β promotes the proliferation and invasion of human glioma cell

Objective To explore the expression level of Glycogen Synthase Kinase-3β( GSK-3β) in glioma cells and its function on cell proliferation and invasion. The underlying mechanism was also investigated. Methods The expression levels of GSK-3β in four glioma cell lines( A172,T98,U87,U251) and normal human astrocytes1800 were analyzed by RT-PCR and Western blotting. The constructed pc DNA3. 1( +)-GSK-3β( r GSK-3β) recombinant vector and PAX3 siRNA were transfected into U87 cells. Cell viability and invasion ability were detected by MTT and Transwell invasion assay,respectively. Results The GSK-3β expression levels were detectable in four glioma cell lines( P 0. 05),but low in the control 1800 cells. Overexpression of GSK-3β in U87 cells increased rates of cell proliferation was assessed by MTT assay and Ki67 expression. The GSK-3β expression promoted cell invasion( P 0. 05). Further analysis suggested that the enhanced PAX3 was responsible for GSK-3β-induced cell proliferation and invasion. These advantages were abolished by inactivation of PAX3 using specific PAX3 siRNA( P 0. 05). Conclusions GSK-3β,via PAX3,promotes cell proliferation and invasion of U87 glioma cells.