In vitrostudy of the anticancer activity of various Doxorubicin-containing dispersions
2018
Introduction:
The
aim of this research was to study the impact of various Doxorubicin (Dox)-containing nanofluids, e.g. single-walled carbon nanotube (SWCNT)+Dox, graphene oxide
(GO)+Dox and Dextran-PNIPAM (copolymer)+Dox mixtures on HeLa
cells (human transformed cervix epithelial cells, as a model for cancer cells)
depending on their concentration.
Methods:
Structural analysis of GO+Dox
complex was accomplished using Hartree-Fock level of theory in 6-31G** basis
set in Gaussian. Dynamic light scattering, zeta-potential, scanning electron
microscopy and confocal laser scanning microscopy were used. The cell viability
was analyzed by the MTT assay.
Results:
The viability of
HeLa cells was studied with the MTT assay after the incubation with various Dox-containing
dispersions depending on their concentration. The size of the particles was
determined by dynamic light scattering. The morphology of the nanoparticles was studied by scanning electron microscopy and their uptake into cells was
visualized by confocal laser scanning microscopy. It
was found that the Dextran-PNIPAM+Dox nanofluid in
contrast to Dox alone showed higher toxicity towards HeLa cells up to 80% after
24 h of incubation, whereas the SWCNT+Dox and GO+Dox nanofluids at the same concentrations protected cells from Dox.
Conclusions:
The importance of Dextran-PNIPAM copolymer as a
universal platform for drug delivery was established, and huge potential of Dextran-PNIPAM+Dox NPs as novel
anticancer agents was noted. Based on the in
vitro study of the SWCNT+Dox
and GO+Dox nanofluids it was concluded that SWCNT and GO NPs can be effective cytoprotectors
against the high toxic drugs.
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