AGROBACTERIUM RHIZOGENES PRI TL-DNA INTEGRATION SYSTEM: A GENE VECTOR FOR LOTUS JAPONICUS TRANSFORMATION

Transfer of T-DNA from binary vectors to transgenic “hairy roots” rely on co-transformation of the T-DNA from the binary vector and the T-DNA from the Ri plasmid into the same plant cell. The frequency of co-transformation varies, so for some experiments an alternative method integrating the gene construct under study diretly into the Ri T-DNA segment may be useful.