Preparation and quality evaluation of ivermectin liposome

【Objective】 The study was to prepare Ivermectin liposome(IVML) and evaluate its quality.【Method】 Modified thin-film dispersion method was adopted to prepare the IVML,which was chosen for influencing factors of formula such as mass ratio of lecithin to cholesterol,mass ratio of Ivermectin(IVM) to lecithin,pH of PBS and of preparation technology such as freeze-thawing times,ultrasonic time,evaporated temperature.These influencing factors were selected by orthogonal design L9(34),of which the optimum factors were adopted to prepare the IVML.High speed freezing centrifugation was used to determine its encapsulation efficiency and its characteristics and stability were evaluated.【Result】 The optimal prescription for preparation of IVML were as follows:m(Lecithin)∶m(Cholesterol)=9∶1,m(IVM)∶m(Lecithin)=1∶10,pH of PBS 7.0,conducting ultrasonic toward 5 minutes,evaporating at 40 ℃,and freezing and thawing 3 times.Its diameter was distributed mainly in 86-115 nm and the average grain size was(91.8±1.5) nm.The encapsulation efficiency of prepared IVML reached(90.71±0.8)%(n=3),and it was stable for heat,but unstable for light.【Conclusion】 The optimal preparation technology of IVML,which was simple and feasible,was obtained.The outlook and content of IVML didn't change clearly and its quality was stable.