Effects of Dengue Virus Infection on Expression of Cytokines in T cells and NK Cells

【Objective】 To investigate the expression of cytokines in T cells and NK cells from human peripheral blood mononuclear cells in response to dengue virus in vitro,and to explore the immune response after dengue virus infection.【Methods】 Flow cytometry was used to detect the phenotype and cytokines of T cells and NK cells from PBMC after DEN-2 infection.【Results】 T cells express CD69,IFN-γ,TNF-α,perforin,and NK cells express CD69,IFN-γ,perforin 18 hours after DEN-2 infection.The numbers of IFN-γ and perforin expressing T cells and perforin expressing NK cells have a positive correlation with dengue virus dose.The numbers of perforin and IL-4 expressing T cells and perforin expressing NK cells increase,whereas the numbers of IFN-γ expressing T cells and NK cells decrease follow infection times.T cells expressing perforin are mainly CD8+T cells.【Conclusion】 With in vitro infection by dengue virus,T cells can express CD69,IFN-γ,TNF-α,perforin and IL-4.And NK cells can express IFN-γ and perforin at the same time.We can conclude that T and NK cells may play an important role in the anti-dengue virus infection or immunopathology.