Optimization of identification technique and germplasm screening of resistance to take-all in wheat.

【Objective】 The purpose of this paper was to find an effective way to identify disease resistance and to screen materials resistant for breeding of resistance to take-all in wheat.【Method】 Identification techniques for take-all were evaluated by using Ningchun 4 as material,and different wheat germplasms were identified by using disc and granule from the pathogen culture for the disease resistance by surveying the percentage of diseased stem and root,and severity level.Disease resistance to different heredity background in 22 wheat materials was identified for screening resistant materials.【Result】 The results showed that the combination of the disc and granule of the fungus(disc+granule) was much better in effects to test take-all fungus to Ningchun 4 than to use the disc or granule separately.The percentage of diseased root,severity level and the percentage of diseased stem were 100.0%,57.3% and 28.6%.There was big difference among the 22 different materials in the disease resistance.Translocation lines Pm97033 showed the highest resistance in reaction than others.The percentage of diseased root and severed level were 11.3% and 5.4%;substitution lines Wan7107 showed medium resistance to the fungus,the percentage of diseased root and severity level were 21.4% and 10.6%;F5 generation of common wheat with TH1 showed higher resistance to take-all fungus than the F5 progenies of common wheat with Aegilops tauschii or the other common wheat varieties.【Conclusion】 The method of disc+granule is a better way to identify resistance to take-all in wheat;TH1 is a good material for the breeding of resistance to take-all in wheat.