Analysis of the Lipid Profile in Patients with Colorectal Cancer in Advanced Stages

Backgrounds: Colorectal (CRC) is one of the main cause of cancer worldwide. The search for noninvasive markersfor diagnosis and monitoring as the use of analytical technologies such as mass spectrometry (MS), which allowed thesearch for lipid metabolites as candidates for probable biomarkers are needed. Objective and Methods: The objectivewas to establish the lipid profile of patients with locally advanced, unresectable or metastatic CRC. Peripheral bloodwas collected from patients with CRC and controls with normal colonoscopy. After lipid extraction, the samples wereprocessed and analyzed in the MALDI TOF / TOF equipment. From the data matrix, the statistical analyzes wereperformed by the principal component analysis methods and the least squares discriminant analysis. The importance ofthe variable in the projection was used to identify the ions that had the greatest discriminatory effect between the groups.Results: Eight lipids were identified as potential biomarkers and a multiple logistic regression model was proposedto calculate the performance of the test where we observed values of AUC 0.87, sensitivity 88.33% and specificity83.78% and for a validation test with 1,000 permutations a p glycerophospholipids and policetidis. The strength of the association between the peak intensities of these lipids andthe presence of CRC make these metabolites candidates for possible biomarkers. The sphingolipid (m / z = 742.98869)could be a biomarker in monitoring patients with CRC. In the survival analysis, three lipids showed a prognostic valuefor colorectal cancer, sphingolipid (m / z = 857.11525) and policetidis (m / z = 876.20796) and glycerophospholipid(m / z = 1031.54773).