Method for industrially producing animal rabies vaccine by utilizing bioreactor

The invention provides a method for industrially producing an animal rabies vaccine by utilizing a bioreactor, comprising the following steps of: (1) sterilizing a micro-carrier and the bioreactor, adding a cell growth solution, inoculating Vero cells for culturing, inoculating rabies viruses after the cell on the micro-carrier forms a compact single layer, and continuously culturing to propagate the viruses; (2) inoculating for 18h and then continuously harvesting a virus solution; (3) carrying out ultrafiltration concentration and virus inactivation on the harvested virus solution; and (4) purifying and inactivating the viruses through a column chromatography method to prepare the vaccine. In the invention, the high density culturing of the cells is carried out by utilizing a bioreactor micro-carrier culturing technology to produce the animal rabies vaccine. Compared with the traditional rotating bottle production method, the automation control degree is high, the production can be monitored in real time, the labor power and the cost are reduced, the production land is less, the scale is easy to expand, the produced virus titer is high, the difference among batches is less, the product quality is stable, and the side reaction is less.