Autoantibodies in the serum of post-stroke patients with different clinical outcome

Introduction/Background Natural autoantibodies (auto-Abs) of IgG class against nervous tissue proteins, as well as corresponding anti-idiotypic antibodies (AIAbs) are presented in serum of each healthy adult and their content are relatively constant among individuals. In opposite, in patients with disorders of the nervous system serum levels of such AIAbs were often found beyond the normal ranges. Characteristic changes of auto-Abs against protein S100, glial fibrillary acidic protein (GFAP), MP-65 protein and nervous growth factor (NGF) and corresponding immune reactivity were investigated in serum samples obtained from patients with ischemic stroke. Samples for investigation were collected at 3, 10, 21 days after stroke and 6 months later. Material and method Forty-two post-stroke patients were examined in acute period of disease and 6 months later. The follow-up was based NIHSS score. Outcomes were assessed with the Bartel Index at 6 month. Severity of post-stroke dementia was defined according to the DSM-IV and NINDS-AIREN. Auto-Abs against S100, GFAP, MP-65 and NGF as well as corresponding AIAbs were determined in patient serum by standard immuno-ferment assay (ELISA). Results In acute period of disease in 100% of post-stroke patients the serum immune reactivity of auto-Abs and AIAbs against proteins S100, GFAP, MP-65 and NGF were deviated compare to the same characteristics in serum samples of healthy persons ( P Conclusion Changes in the serum content of auto-Abs against nervous tissue proteins and corresponding AIAbs in post-stroke patients may reflect intensity of reparative processes and probably could be used for prognostic aims of rehabilitation program success.