Characterization of a cryptic plasmid from endophytic Enterobacter agglomerans isolated from Amazon's Copaifera multijuga

A small plasmid named pEA1 was isolated from Enterobacter agglomerans, an endophytic bacterium colonizing Copaifera multijuga (copaiba). The pEA1 sequence was determined by using the primer walking strategy and was deposited in GenBank (accession number DQ659147). pEA1 is a circular molecule with a length of 2,545 bp. Sequence analysis showed this plasmid has a GC content of 34% (Guanine and Citosine content in DNA sequence) and contains four open reading frames (ORFs) with capacity of encoding more than 100 amino acids. The ORF1, ORF2, and ORF3 showed homology to mobilization proteins. ORF4 showed homology to protein involved in replication in a plasmid originating from Pectobacterium atrosepticum.