Oligomer synthesis and DNA/RNA recognition properties of a novel oligonucleotide backbone analog: Glucopyranosyl nucleic amide (GNA)

Abstract A general solid phase synthesis is described for the construction of novel amide linked oligonucleotide analogs having the glucopyranosyl configuration via solid phase N-Fmoc-type peptide chemistry. The selective binding to DNA and RNA sequences by these oligomers is characterized by measurement of melting temperatures and related thermodynamic constants.