Bradykinin induces generation of reactive oxygen species in bovine aortic endothelial cells.

We investigated the effects of bradykinin on intracellular oxidative stress in bovine aortic endothelial cells using a hydroperoxide-sensitive fluorescent dye, 2',7'-dichlorofluorescein (DCFH), and a laser scanning confocal microscope. Bradykinin induced an immediate increase in intracellular Ca 2+ concentration, and stimulated the oxidation of DCFH in cultured endothelial cells